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UV spectrophotometric method development and validation for estimation of ketoconazole in bulk and pharmaceutical dosage form

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Original Article

Author Details : Shrishail Ghurghure, Shruti Satish Garad*, Shubhangi Birajdar

Volume : 5, Issue : 2, Year : 2023

Article Page : 71-74

https://doi.org/10.18231/j.ctppc.2023.016

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Abstract

Purpose: For the determination of ketoconazole in bulk and pharmaceutical dosage form in accordance with ICH guidelines, a new, economical, precise, sensitive, linear, accurate, and quick UV-Spectrophotometric approach has been developed in Methylene chloride.
Materials and Methods: The UV-visible spectrum of Ketoconazole was examined to determine its maximum absorption wavelength that is ? max at 255.2 nm. Linearity, accuracy, precision and robustness tested for this procedure.
Results: Ketoconazole was found to have a maximum absorbance at 255.2 nm. The regression coefficient for the concentration range of 5–25 µg/ml. Ketoconazole. LOD and LOQ were determined to be 0.0225 and 0.75 µg/ml, respectively. The procedure was successfully used on ketoconazole in commercial formulation, and the outcomes were in good agreement with label claims.
Conclusion: Depending on the results, the given method can be successfully applied of Ketoconazole in bulk and Pharmaceutical dosage form.

Keywords: UV­Visible Spectrophotometer, Ketoconazole, Methylene chloride

How to cite : Ghurghure S, Garad S S, Birajdar S, UV spectrophotometric method development and validation for estimation of ketoconazole in bulk and pharmaceutical dosage form. Curr Trends Pharm Pharm Chem 2023;5(2):71-74

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