Introduction
Antimicrobial Agent 1 is defined as agent that prevents the growth or propagation of bacteria or microorganisms including viable spores on inanimate objects.
Synthesis was performed for compounds VG1 and VG2 which are chemically 4-nitro-benazmides. VG2 compound is shown in the figure below which is chemically N-(propan-2-yl)-4-nitrobenzamide. The compounds were confirmed by TLC, FT-IR and 1H-NMR.
The spectral data and synthesis procedures of VG1 and VG2 had been reported in Part-I of this paper. 2
We here report the antimicrobial activity of the compound VG2 conducted in our laboratories.
Materials and Methods
Compound VG2
This is chemically N-(-propan-2-yl)-4-nitrobenzamide and has been synthesized by known literature procedure and published in Part-I of this paper.1
Antimicrobial activity/ screening
Test Solution: 20 nano molar of compound VG2 was dissolved in 3.0 grams of ethanol and stored.
Microbial culture
Culture Growth Medium was inoculated with soil water and incubated for the growth of the bacterial load for 72 hrs. Rather than isolating a pure strain of bacteria, microbial culture load was used and inoculated further to give subculture. Further, this culture was used for antimicrobial screening for further testing of VG2. The process involved standard, vehicle and control. The standard being Brand X disinfectant, control being Water and vehicle being ethanol. Test being the compound VG2 dissolved in ethanol, 20nmoles in strength and being evaluated. Zone of inhibition (ZOI) was measured in centimetres.
Results and Discussion
As per Table 1, the compound VG2 showed antimicrobial properties as compared to the standard branded antimicrobial (X) and Vehicle used in the test.
